UPTAKE OF P 32 IN RESTING CELLS OF CLOSTRIDIUM PERFRINGENS

Uptake of P32 in resting cells of Clostridium perfringens.

Previous studies on resting cell metabolism of microorganisms relating to p32 incorporation into the intact cell have been carried out by Hotchkiss (1956) for micrococci, lactobacilli, and streptococci, and by Juni et al. (1948) for yeast. These studies in the main were concerned with turnover of various intracellular phosphoruscontaining compounds during conditions under which the organism was...

Preparation of Standard Clostridium perfringens Antitoxin in the Sheep

Cloning of Clostridium perfringens iota toxin gene in Escherichia coli

Iota toxin is produced by Clostridium perfringens type E. This toxin causes antibiotic-associated enterotoxemia in lambs and calves. Iota toxin is a binary toxin that has two components including Ia (the enzyme component) and Ib (the binding component). Ib binds to the surface receptor of target cells and translocate Ia into the cytosol of cells. The aim of this study was to clone toxigenic epi...

Cellular uptake of the Clostridium perfringens binary iota-toxin.

The binary iota-toxin is produced by Clostridium perfringens type E strains and consists of two separate proteins, the binding component iota b (98 kDa) and an actin-ADP-ribosylating enzyme component iota a (47 kDa). Iota b binds to the cell surface receptor and mediates the translocation of iota a into the cytosol. Here we studied the cellular uptake of iota-toxin into Vero cells. Bafilomycin ...

Production and standardization of polyvalent Clostridium perfringens vaccine in Iran